TY - UNPD A1 - Gierer, Alfred A1 - Mundry, K. W. T1 - Production of mutants of Tobacco Mosaic Virus by chemical alteration of its ribonucleic acid in vitro N2 - The generation of viral mutants in vitro was demonstrated by treatment of the isolated RNA of Tobacco Mosaic Virus by nitrous acid. This agent causes deaminations converting cytosine into uracil, and adenine into hypoxanthine. Our assay for mutagenesis was the production of local lesions on a tobacco variety on which the untreated strain produces systemic infections only. A variety of different mutants are generated in this way. Quantitative analysis of the kinetics of mutagenesis leads to the conclusion that alteration of a single out of the 6000 nucleotides of the viral RNA is sufficient for causing a mutation. KW - TMV KW - RNA KW - desamination KW - mutagenesis KW - mutation in vitro Y1 - 2006 UR - https://edoc.bbaw.de/frontdoor/index/index/docId/185 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b4360-1001026 UR - http://edoc.bbaw.de/oa/articles/rek60UNlIJS9E/PDF/29sZBHzpOuqto.pdf ER -