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Production of mutants of Tobacco Mosaic Virus by chemical alteration of its ribonucleic acid in vitro

  • The generation of viral mutants in vitro was demonstrated by treatment of the isolated RNA of Tobacco Mosaic Virus by nitrous acid. This agent causes deaminations converting cytosine into uracil, and adenine into hypoxanthine. Our assay for mutagenesis was the production of local lesions on a tobacco variety on which the untreated strain produces systemic infections only. A variety of different mutants are generated in this way. Quantitative analysis of the kinetics of mutagenesis leads to the conclusion that alteration of a single out of the 6000 nucleotides of the viral RNA is sufficient for causing a mutation.

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Author:Alfred Gierer, K. W. Mundry
Document Type:Working Paper
Date of Publication (online):2006/11/06
Release Date:2006/11/06
Tag:RNA; TMV; desamination; mutagenesis; mutation in vitro
Source:Nature : a weekly journal of science ; 182, S. 1457-1458
Institutes:BBAW / Veröffentlichungen von Akademiemitgliedern
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik