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Structure and biological function of ribonucleic acid from Tobacco Mosaic Virus

  • Within the sedimentation diagram of infective RNA preparations isolated from Tobacco Mosaic Virus, undegraded molecules form a sharp peak with a molecular weight corresponding to the total RNA content of the virus particle. Degradation kinetics by ribonuclease is of the linear, single-target type, indicating that the RNA is single-stranded. The intact RNA of a virus particle thus forms one big single-stranded molecule. Quantitative evaluation of the effect degradation by RNA-ase on the infectivity of the RNA shows that the integrity of the entire molecule is required for its biological activity.

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Metadaten
Author:Alfred Gierer
URN:urn:nbn:de:kobv:b4360-1001017
URL:http://edoc.bbaw.de/oa/articles/rePbSUW9jsXYU/PDF/24aF8Y77EwS8s.pdf
Document Type:Working Paper
Language:English
Date of Publication (online):2006/11/06
Publishing Institution:Berlin-Brandenburgische Akademie der Wissenschaften
Release Date:2006/11/06
Tag:RNA; TMV; infectivity; single strand
Source:Nature : a weekly journal of science ; 179, S. 1297-1299
Institutes:BBAW / Veröffentlichungen von Akademiemitgliedern
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik
        
            
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