Structure and biological function of ribonucleic acid from Tobacco Mosaic Virus
- Within the sedimentation diagram of infective RNA preparations isolated from Tobacco Mosaic Virus, undegraded molecules form a sharp peak with a molecular weight corresponding to the total RNA content of the virus particle. Degradation kinetics by ribonuclease is of the linear, single-target type, indicating that the RNA is single-stranded. The intact RNA of a virus particle thus forms one big single-stranded molecule. Quantitative evaluation of the effect degradation by RNA-ase on the infectivity of the RNA shows that the integrity of the entire molecule is required for its biological activity.
Author: | Alfred Gierer |
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URN: | urn:nbn:de:kobv:b4360-1001017 |
URL: | http://edoc.bbaw.de/oa/articles/rePbSUW9jsXYU/PDF/24aF8Y77EwS8s.pdf |
Document Type: | Working Paper |
Language: | English |
Date of Publication (online): | 2006/11/06 |
Publishing Institution: | Berlin-Brandenburgische Akademie der Wissenschaften |
Release Date: | 2006/11/06 |
Tag: | RNA; TMV; infectivity; single strand |
Source: | Nature : a weekly journal of science ; 179, S. 1297-1299 |
Institutes: | BBAW / Veröffentlichungen von Akademiemitgliedern |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik |