Function of aggregated reticulocyte ribosomes in protein synthesis
- Applying mild methods of preparation, part of the ribosomes of rabbit reticulocytes are found in aggregates (later called polyribosomes) of up to six ribosomal units. Upon treatment with RNA-ase, they desintegrate into single ribosomes. The fast-sedimenting aggregates are found to be more active in protein synthesis in terms of incorporation of radioactive amino acids, whereas the single ribosomes are more receptive to stimulation by the artificial messenger RNA poly-U. The findings indicate that the linkage of ribosomes into aggregates is due to the messenger RNA. They support a tape-reading mechanism of protein synthesis whereby growth of the peptide chain is accompanied by shifting the active site of the ribosome from one coding group of nucleotides of the messenger RNA to the next.
| Verfasserangaben: | Alfred Gierer |
|---|---|
| URN: | urn:nbn:de:kobv:b4360-100730 |
| URL: | http://edoc.bbaw.de/oa/books/reM2apjEnfctQ/PDF/29fMUqxcYCU.pdf |
| Dokumentart: | Wissenschaftlicher Artikel (in einer Zeitschrift) |
| Sprache: | Englisch |
| Datum der Veröffentlichung (online): | 30.10.2006 |
| Veröffentlichende Institution: | Berlin-Brandenburgische Akademie der Wissenschaften |
| Datum der Freischaltung: | 30.10.2006 |
| Freies Schlagwort / Tag: | Polyribosomes; Polysomes; Reticulocytes; messenger RNA; protein synthesis |
| Quelle: | Journal of Molecular Biology ; 6, S. 148-157 |
| Institute: | BBAW / Veröffentlichungen von Akademiemitgliedern |
| DDC-Klassifikation: | 000 Wissenschaft, Allgemeines / 00 Informatik, Wissen, Systeme / 000 Informatik, Informationswissenschaft, allgemeine Werke |
